Founded in early 2005, StratiCELL is a leading company assisting the cosmetic, pharmaceutical and chemical industries to the safety and efficacy evaluation of their actives and products by means of in vitro models and methods in a GLP-compliant environment.
StratiCELL is a skin model manufacturer and a project-driven services provider for in vitro testing, with a particular emphasis on testing of skin care actives and products
Epidermal Differentiation
The stratum corneum from epidermis provides a physical and permeability barrier, which is continuously regenerated by terminally differentiating keratinocytes. It is formed by the subcellular organization of epidermal proteins and lipids into a two-compartment system with a continuous extracellular lipid matrix surrounding the lipid-depleted, protein-enriched corneocytes.
The StratiCELL reconstituted epidermis undergoes a similar epidermal differentiation process, as in human skin.
Cell proliferation, differentiation and death occur sequentially and each process is characterized by the expression of specific proteins.
In Vitro biological endpoints for Epidermal differentiation
- Localization and/or quantification of structural proteins involved in the epidermal differentiation, by immuno-fluorescence or immuno-histology, in StratiCELL reconstituted epidermis (K10, filaggrin, loricrin...)
- Gene expression profiling of key genes involved in epidermal differentiation
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Immunofluorescence of Filaggrin/Keratin 10 in sections of StratiCELL® reconstituted human epidermis EPI/001 |
