In vitro reconstituted epidermis

 

StratiCELL Reconstituted Human Epidermis (RHE/001) is produced from a pool of human keratinocytes from different donors ensuring the highest level of standardization and reproducibility of the test results.

Cells are seeded on polycarbonate filters grown at the air-liquid interface with appropriate cell culture medium, free of animal serum. After two weeks of differentiation, a full epidermis is reconstituted.

The StratiCELL reconstituted epidermis is very similar to human skin in terms of morphology, of differentiation markers and of functional characteristics. Indeed, StratiCELL reconstituted human epidermis model consists of a living, epidermal tissue fully stratified and differentiated as demonstrated by the expression profile of multiple differentiation markers as keratin 14, keratin 10, tight junction proteins, corneodesmosin, involucrin, etc. 

This model represents a relevant tool for safety and dermato-cosmetic activity testing, for penetration and absorption studies and for providing insights into mechanisms of action of an active or product.

The use of melanocytes from Black or Caucasian donors also enable the reconstitution of dark tanned and light tanned pigmented tissues, respectively (MEL/001)

This model represents a relevant tool to investigate and characterize molecular pathways involved in skin melanogenesis, de- and pro-pigmentation as well as to study new active ingredients. MEL/001 is also used to evaluate photo-protection

Custom-made models

StratiCELL's roots being situated between basic and applied research, R&D and innovation commitments are of key importance in the company's philosophy. This is illustrated by custom-made models such as reconstituted epidermis from skin biopsies or suction blistering. StratiCELL owns indeed a large library of more than 100 keratinocyte cultures from young, old, diseased individuals. These models represent unique tools to investigate and characterize molecular pathways involved in skin diseases (psoriasis, dermatitis etc.) as well as to validate new drug acting on the skin pathology or to compare skin reaction depending on the age, an interesting endpoint which can be studied.