Founded in early 2005, StratiCELL is a leading company assisting the cosmetic, pharmaceutical and chemical industries to the safety and efficacy evaluation of their actives and products by means of in vitro models and methods in a GLP-compliant environment.
StratiCELL is a skin model manufacturer and a project-driven services provider for in vitro testing, with a particular emphasis on testing of skin care actives and products
StratiCELL EPI/001
The StratiCELL® Reconstituted Human Epidermis is produced from human keratinocytes originating from foreskin tissue from a pool of different donors. This unique feature in our manufacturing procedure ensures the highest level of standardization and reproducibility of the test results.
Cells are seeded on polycarbonate filters (standard size 0.63 cm2), grown at the air-liquid interface in an appropriate cell culture medium in an insert (figure 1a). After two weeks of differentiation, a full epidermis is reconstituted.
The StratiCELL® reconstituted epidermis is very similar to human skin. In terms of morphology for instance, the well-characterized layers of a differentiated epidermis, -the basal, spinous, granular and cornified layers (cited from bottom to top)-, are present in the in vitro model as well as observed by a histology assessment (figure 1b). With on the top the appearance of terminally differentiated keratinocytes that form the protective stratum corneum layer. This morphology is accompanied with a highly similar expression profile of different differentiation markers in the reconstituted epidermis in comparison to human skin (such as keratins 10 & 14, involucrin, figure 2). In terms of functional characteristics, the in vitro epidermal model retains, its capacity to synthesize inflammatory cytokines in response to irritating or sensitizing agents.
Each batch of reconstituted epidermis is submitted to quality control (viability and histoloy). 
Normal Human reconstituted epidermis (0,63 cm2)
Figure 1b: The StratiCELL® Reconstituted Human Epidermis (EPI/001) H/E histological staining. Appearance of a fully differentiated epidermis with the presence of the basal, spinous, granular and the cornified layers with the stratum corneum
Figure 2: Visualization of differentiation marker in the StratiCELL® Reconstituted Human Epidermis (EPI/001), compared to human skin. In green,
the protein of interest, cell nuclei are counter-stained in blue by DAPI staining.
StratiCELL® Reconstituted Human Epidermis is commercially available, please contact
This e-mail address is being protected from spambots. You need JavaScript enabled to view it
for prices and delivery dates.
In Vitro Applications of StratiCELL® Reconstituted Human Epidermis
- In vitro dermal toxicity assessment (evaluation skin corrosion, irritation, phototoxicity,...);
- Screening for biological activities in the skin after treatment with skin care substances or products (skin barrier function, sun protection,...);
- In vitro skin absorption and penetration studies.
