Anti-inflammation and calming
Anti-inflammation and calming properties of compounds can be addressed by following the secretion profile of multiple cytokines like IL1α, IL-1β, IL-6, IL-8, TNFα and of prostaglandins/PGE2 and LTB4.
Inflammatory mediators are induced by PMA, UV challenge, calcium ionophore A23187, or arachidonic acid, depending on the model and the targeted cytokine/protaglandin.
Models include primary keratinocytes and in vitro reconstituted epidermis, suitable for systemic or topical application respectively.
Screening tools for atopic dermatitis